cd44 apc-cy7 Search Results


cd44 apc cy7  (Novus Biologicals)
92
Novus Biologicals cd44 apc cy7
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Cd44 Apc Cy7, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-cd44-apc-cy7 (im7)  (Thermo Fisher)
90
Thermo Fisher anti-cd44-apc-cy7 (im7)
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Anti Cd44 Apc Cy7 (Im7), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apc-cy7 anti-mouse cd44  (Becton Dickinson)
90
Becton Dickinson apc-cy7 anti-mouse cd44
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Apc Cy7 Anti Mouse Cd44, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd105-apc antibody  (Nordic BioSite)
90
Nordic BioSite cd105-apc antibody
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Cd105 Apc Antibody, supplied by Nordic BioSite, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd4-fitc antibody  (Thermo Fisher)
90
Thermo Fisher cd4-fitc antibody
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Cd4 Fitc Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd44 apc cy7  (Cytek Biosciences)
93
Cytek Biosciences cd44 apc cy7
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Cd44 Apc Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-cd44-apc/cy7  (Thermo Fisher)
90
Thermo Fisher anti-cd44-apc/cy7
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Anti Cd44 Apc/Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd117 (c-kit) mouse apc 2b8 antibody  (Thermo Fisher)
90
Thermo Fisher cd117 (c-kit) mouse apc 2b8 antibody
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Cd117 (C Kit) Mouse Apc 2b8 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3 efluor450  (Thermo Fisher)
90
Thermo Fisher cd3 efluor450
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Cd3 Efluor450, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody cd3ε f506  (Thermo Fisher)
90
Thermo Fisher antibody cd3ε f506
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Antibody Cd3ε F506, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd11b apc-cy7 m1/70 rat da  (Becton Dickinson)
90
Becton Dickinson cd11b apc-cy7 m1/70 rat da
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Cd11b Apc Cy7 M1/70 Rat Da, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd44 apc cy7  (Thermo Fisher)
86
Thermo Fisher cd44 apc cy7
Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells <t>(CD4+CD62L+CD44+</t> and <t>CD8+CD62L+CD44+),</t> 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.
Cd44 Apc Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells (CD4+CD62L+CD44+ and CD8+CD62L+CD44+), 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.

Journal: Acta Biomaterialia

Article Title: Multipolymer microsphere delivery of SARS-CoV-2 antigens

doi: 10.1016/j.actbio.2022.12.043

Figure Lengend Snippet: Cellular immune response to MS-Ag administration in SD rats. SD rats (average body weight = 270 g) were each injected with a single intramuscular dose of 6 µg/g of body weight of chemically inactivated SARS-CoV-2 loaded MS (MS-Ag). From SD rats injected with MS-Ag, empty MS or saline, T-cell subsets of splenocytes were analyzed at 7 and 28 dpi by flow cytometry. (a-b) Percentage population of activated T-cells (CD4+CD69+ and CD8+CD69+), 28 dpi. (c-d) Percentage population of TCM - cells (CD4+CD62L+CD44+ and CD8+CD62L+CD44+), 28 dpi. Intracellular cytokine staining and flow cytometric analysis of CD4+ T-cell subset expressing (e) IFNγ at 7 dpi, (f) IFNγ at 28 dpi, (g) IL4 at 28 dpi, and (h) IL17 at 28 dpi. Statistical analysis was done by One-way ANOVA followed by Newman/Keul's post-hoc analysis for multiple comparisons, (*P < 0.05; **P < 0.01; ***P < 0.001). Abbreviations: MS, multipolymer microsphere; Ag, antigen; IFNγ, Interferon-gamma; IL, Interleukin; %, percentage of the parent population.

Article Snippet: For extracellular staining, 2 × 10 6 of spleen cells were first stained with LIVE/DEADTM Fixable Blue Dead Cell Stain Kit in PBS for 30 min. After saline washes, cells were then stained with CD3-PE (201412, Biolegend), CD4-BUV737 (741770, BD Biosciences), CD8a-BV650 (740514, BD Biosciences), CD62L-eFluor660 (50-0623-82), CD44-APC Cy7 (NB600-1317APCCY7, Novus Biologicals), CD69-AlexaFluor488 (bs-2499R-A488) antibodies to detect surface antigens.

Techniques: Injection, Tandem Mass Spectroscopy, Saline, Flow Cytometry, Staining, Expressing